Journal
FOOD CHEMISTRY
Volume 105, Issue 4, Pages 1504-1511Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2007.05.032
Keywords
grape seeds; catechin; epicatechin; degradation; water activity; antioxidant activity
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Storage effect on antioxidant content and capacity of grape seeds under different a, conditions (a(w) 0.33; 0.53; 0.75/50 days, 25 degrees C) was examined. Total phenol content (determined by the Folin-Ciocalteu method) decreased during storage though changes were trivial for samples stored at 33% or 53% RH. High level of humidity (75%) accelerated degradation and resulted in a similar to 50% reduction of total phenol content. Minor loss of the DPPH radical scavenging activity (%RSA) of the extracts was observed. Catechin and epicatechin content monitored by RP-HPLC was reduced during storage, particularly at 75% RH. Epicatechin content proved to be less sensitive to water activity conditions than catechin content. Results of various in vitro assays (Folin-Ciocalteu, FRAP, DPPH, ABTS, CBA, ORAC and copper induced liposome oxidation) did not support difference in terms of resistance to oxidation. Based on the continuous release of gallic acid, our finding was related to hydrolytic reactions. Control of a, of grape seeds can be of practical importance for the wine industry. (c) 2007 Elsevier Ltd. All rights reserved.
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