Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
Volume 146, Issue 1, Pages 124-130Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpa.2006.09.017
Keywords
cholecystokinin; gastrin; pancreas; pyloric caeca; trypsin; chymotrypsin; in vitro assays; proteolytic enzyme secretion; temperature; yettowtail (Seriola quinqueradiata)
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The Immoral control of secretion of the proteolytic enzymes trypsin and chymotrypsin was studied in yellowtail (Seriola quinqueradiata). In vitro trials were performed to investigate the effects of cholecystokinin (CCK) and two commercially available gastrin peptides. Isolated preparations of pyloric caeca/pancreas release trypsin and chymotrypsin when incubated with cholecystokinin (CCK) at 10 mu M and gastrin I (G1) at 50 mu M after 15 min of incubation. On the other hand, G1 at 10 mu M and gastrin-related peptide (G2) did not enhance trypsin and chymotrypsin secretion. The studies concerning the CCK effects at different incubation temperatures have shown that trypsin and chymotrypsin secretion at 25 degrees C was stimulated by CCK after 15 min, while at 10, 15 and 20 degrees C the stimulatory effects of CCK were observed only after 30 min of incubation. The CCK effects were increased at higher incubation temperatures and longer incubation periods. (c) 2006 Elsevier Inc. All rights reserved.
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