Journal
FOOD CHEMISTRY
Volume 101, Issue 1, Pages 308-313Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2006.01.038
Keywords
chitosan; molecular weight; lipase; antioxidative activity
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Antioxidant activity of chitosans of different molecular weights (30, 90 and 120kDa chitosan) in salmon (Salmo salar) was investigated. The progress of oxidation was monitored by employing the 2-thiobarbituric acid-reactive substances (TBARS) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assays. In general, all chitosans exhibited antioxidative activities in salmon. The addition of chitosans to salmon reduced lipid oxidation for seven days of storage. The TBARS values of salmon containing chitosan were significantly lower than those of the control (p < 0.01). At 0.2% (w/v) and 0.5% (w/v) concentrations, the TBARS with chitosan addition was decreased by 75% and 45%, respectively, over 15 days. At 1% concentration, the TBARS value with native chitosan addition was decreased by 32% after 15 days of storage. 90 kDa chitosan showed an increased DPPH free radical-scavenging activity with increasing concentration in the range of 0.2-1% (w/v). The free radical-scavenging activity of the 0.2 mM DPPH solution was saturated by 30 kDa chitosan at a concentration of >= 0.7% (w/v), resulting in a strong antioxidant activity of approximately 85%. This was comparable to the DPPH free radical-scavenging activity of BHT. (c) 2006 Elsevier Ltd. All rights reserved.
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