Journal
FOOD CHEMISTRY
Volume 101, Issue 1, Pages 345-350Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2005.12.056
Keywords
biotransformation; industrial residues; R-(+)-limonene; R-(+)-alpha-terpineol; fusarium oxysporum
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The use of two agro-residues (liquid cassava waste and orange essential oil) in the biotransformation of R-(+)-limonene was investigated. The main components of orange essential oil were determined by GC-MS and R-(+)-limonene was shown to be the predominant constituent, accounting for more than 94% of the total content. Cassava wastewater proved to be a suitable substrate for mycelial growth, leading to good, rapid growth with all the fungal strains tested, reaching 29.4 g/l (dry weight) after 3 days of growth (Penicillium sp. 2025). The best R-(+)-alpha-terpineol yields were achieved when the strains were grown in cassava media and the mycelia then transferred to a new flask containing mineral medium and orange essential oil as the sole C- and energy source. One of the strains tested, Fusarium oxysporum 152B, converted R-(+)-limonene to R-(+)-alpha-terpineol, yielding nearly 450 mg/l after 3 days of transformation. Growth in the presence of a solution of 1% orange essential oil in decane did not increase the transformation yields. (c) 2006 Elsevier Ltd. All rights reserved.
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