Journal
MOLECULAR IMMUNOLOGY
Volume 44, Issue 11, Pages 2933-2942Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.molimm.2007.01.015
Keywords
acute phase reactants; transcription factors; gene regulation; molecular biology
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Funding
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [T32DK007319] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [R01AG002467] Funding Source: NIH RePORTER
- NIA NIH HHS [AG 02467] Funding Source: Medline
- NIDDK NIH HHS [T32 DK 007319-28] Funding Source: Medline
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C-reactive protein (CRP) is a plasma protein primarily synthesized in the liver following inflammatory stimuli as part of the acute phase response. Expression of CRP is tightly regulated in hepatocytes. Normally very little CRP mRNA is transcribed, but inflammatory stimuli are followed by a dramatic increase in mRNA synthesis and accumulation. Interleukins -6 and 1 (IL-6 and IL-1) are believed to be the major cytokines responsible for induction of acute phase protein biosynthesis. We previously demonstrated that in vivo c-Rel plays a novel regulatory role in that it appears to be in complex with C/EBP beta when C/EBP beta is bound to the CRP gene promoter following cytokine stimulation, but is not itself bound to DNA. In this study we found that recombinant c-Rel((1-300)) (truncated c-Rel protein missing the transactivation domain) increased the affinity of recombinant C/EBP beta for a CRP-derived C/EBP site (-53) at least 10-fold. This effect was independent of a previously described p50 binding site at -43 and of binding of c-Rel to DNA. C/EBP beta and c-Rel((1-300)) were found to physically interact in solution, and overexpression of c-Rel (either full length or truncated (1-300)) in the presence of overexpressed C/EBP beta stimulated CRP transcription. We conclude that c-Rel((1-300)) binding to C/EBP beta increases the affinity of C/EBP beta for the C/EBP binding site at -53 on the CRP promoter, and that the transactivation domain of c-Rel is not necessary for this effect, which depends on protein: protein contacts with C/EBP beta. (c) 2007 Elsevier Ltd. All rights reserved.
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