Journal
GENETICS
Volume 177, Issue 1, Pages 615-629Publisher
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.107.076216
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Funding
- Biotechnology and Biological Sciences Research Council [G18877] Funding Source: Medline
- Medical Research Council [G8225539] Funding Source: Medline
- MRC [G8225539] Funding Source: UKRI
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We describe a second-gene ration deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering similar to 77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verified a set of 209 deletion-bearing fly stocks. In addition to deletions, we demonstrate how the P elements may also be used to generate a set of custom inversions and duplications, particularly useful for balancing difficult regions of the genome carrying haplo-insufficient loci. We describe a simple computational resource that facilitates selection of appropriate elements for generating custom deletions. Finally, we provide a computational resource that facilitates selection of other mapped FRT-bearing elements that, when combined with the DrosDel collection, can theoretically generate over half a million precisely mapped deletions.
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