Journal
NANO LETTERS
Volume 7, Issue 6, Pages 1680-1685Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl070668c
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Funding
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR005969] Funding Source: NIH RePORTER
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R01HG000371, R01HG003713] Funding Source: NIH RePORTER
- NCRR NIH HHS [P41 RR05969] Funding Source: Medline
- NHGRI NIH HHS [R01 HG003713, R01 HG003713-02, R01 HG003713A] Funding Source: Medline
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We have discovered a voltage threshold for permeation through a synthetic nanopore of dsDNA bound to a restriction enzyme that depends on the sequence. Molecular dynamic simulations reveal that the threshold is associated with a nanonewton force required to rupture the DNA-protein complex. A single mutation in the recognition site for the restriction enzyme, i.e., a single nucleotide polymorphism (SNP), can easily be detected as a change in the threshold voltage. Consequently, by measuring the threshold voltage in a synthetic nanopore, it may be possible to discriminate between two variants of the same gene (alleles) that differ in one base.
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