Journal
NANO LETTERS
Volume 7, Issue 3, Pages 754-760Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl063020t
Keywords
-
Categories
Funding
- NATIONAL CANCER INSTITUTE [R21CA095393] Funding Source: NIH RePORTER
- NCI NIH HHS [R21CA95393-01] Funding Source: Medline
Ask authors/readers for more resources
The effects of four types of fullerene compounds (C-60, C-60-OH, C-60-COOH, C-60-NH2) were examined on two model microorganisms (Escherichia coli W3110 and Shewanella oneidensis MR-1). Positively charged C-60-NH2 at concentrations as low as 10 mg/L inhibited growth and reduced substrate uptake for both microorganisms. Scanning electron microscopy (SEM) revealed damage to cellular structures. Neutrally charged C-60 and C-60-OH had mild negative effects on S. oneidensis MR-1, whereas the negatively charged C-60-COOH did not affect either microorganism's growth. The effect of fullerene compounds on global metabolism was further investigated using [3-C-13]L-lactate isotopic labeling, which tracks perturbations to metabolic reaction rates in bacteria by examining the change in the isotopic labeling pattern in the resulting metabolites (often amino acids).(1-3) The C-13 isotopomer analysis from all fullerene-exposed cultures revealed no significant differences in isotopomer distributions from unstressed cells. This result indicates that microbial central metabolism is robust to environmental stress inflicted by fullerene nanoparticles. In addition, although C-60-NH2 compounds caused mechanical stress on the cell wall or membrane, both S. oneidensis MR-1 and E. coli W3110 can efficiently alleviate such stress by cell aggregation and precipitation of the toxic nanoparticles. The results presented here favor the hypothesis that fullerenes cause more membrane stress(4-6) than perturbation to energy metabolism.(7)
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available