Journal
ORAL ONCOLOGY
Volume 43, Issue 1, Pages 60-66Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.oraloncology.2006.01.005
Keywords
oral squamous cell carcinoma; fibroblast growth factor receptor 1 (FGFR1); matrix-CGH; array CGH; tissue microarray analysis
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Chromosomal aberrations are known to have an impact on the initiation and progression of oral squamous cell carcinoma (OSCC), but individual genes involved in OSCC pathogenesis are poorly described. To elucidate the molecular events underlying oral carcinogenesis, a set of primary OSCC were screened for distinct genetic imbalances by means of array-based comparative genomic hybridisation. For this, a DNA array was used containing 812 genomic targets including oncogenes, tumour-suppressor genes and chromosomal regions frequently altered in human neoplasms. The most frequent aberrations were amplification of MYC, EGFR, CCND1 and PIK3CA, whereas deletions affected TRAILR1 and ATM. Furthermore, a distinct high-level amplification of the fibroblast growth factor receptor 1 (FGFR1) locus was detected in two cases. Detailed FISH analysis on OSCC tissue microarray sections revealed amplification prevalence for FGFR1 of 17.4% (16/92). Furthermore, FGFR1 protein analysis by immunohistochemistry on a TMA containing 178 OSCC found a high FGFR1 expression in tumours of early t-stadiumand UICC stage (T1/2 vs. T3/4: p = 0.002; SI-II vs. S III-IV: p = 0.048). Our results indicate that an increase in FGFR1 expression contributes to oral carcinogenesis at an early stage of development. (c) 2006 Elsevier Ltd. All rights reserved.
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