Journal
BIOMATERIALS
Volume 28, Issue 3, Pages 393-399Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2006.09.002
Keywords
calcium sulfate; bone graft; gene expression; osteoinduction; bone healing
Ask authors/readers for more resources
Recently, calcium sulfate dihydrate has been demonstrated Lis safe biodegradable osteoconductive bone void filler. However. its exact mechanism of action on bone cells is yet unknown. In this study, the influence of gypsum on gene expression and proliferation of MC3T3-E1 mouse pre-osteoblastic cells was investigated. Cells were cultured on gypsum disc, slice, polymethylmethacrylate (PMMA), or plastic culture plate for 15 days. Cell viability, alkaline phosphatase (ALP) activity and expression profile of 15 genes involved in bone metabolism were measured in cultures. Cell proliferation on gypsum was increased by almost 2-fold, while ail inhibitory effect of PMMA on proliferation rate of osteoblasts was noted. Cells cultured on gypsum disc surface exhibited an increased ALP activity and markedly different gene expression profile. Quantitative real-time PCR data indicated the expression of genes that might provide a basis for an osteoinductive potential. MC3T3-EI cells expressed genes typical of bone fracture healing like type 11 collagen and fibronectin 1. These effects might be related to the calcium content of gypsum and mediated likely via SMAD3. Our results suggest that gypsum call support new bone formation by its calcium content and modulatory effect on gene expression profile of bone cells. (c) 2006 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available