Journal
NEURON GLIA BIOLOGY
Volume 3, Issue -, Pages 269-279Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S1740925X08000045
Keywords
muscarinic receptors; proliferation; neurotransmitter; axon-glia interaction; sciatic nerve
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Cultures of schwalla cells froill ncoaatal rat sciatic aenes ivere treated with acety1cholilic agonists tvid the cjj cts oil cell Prolificratiou cniluatcd. 3[HI-thyaiidiac incorporation shows that accty1choliac (ACh) receptor agoiiists ilillibit cell proliftratioti, aiid FACS analysis deinoustratcs ccll-cyclc arrest and accunlulatioii of cells ill the Gi phase. The use of arecaiditie, a selective agouist of niuscariiiic M2 receptors reveals that this effect depends nictitily oil jW2 receptor acth,atioii. The arecaidine i1cpetialci i t- block ill Gi is rci,ersible becausc reinoval of arecaidiue froai the culture iiieditaii mduccs progrcssioil to the S phase. The block of the Gj-S transitioil is also characterized by inodulatiou of the expressioti ofscwrol cell-cycic inarkcrs. Morcoi,cr, trcatnicat ivith ACh receptor agmist causes both a decreasc ill the PCNA prolcin lci,cls ill Schivaiiii cell iniclci aad all iiicreasc ill P27 Olld P53 proteins. Filially, iinnnuio-elcctrou inicroscopy dcalonStl-atCS that M2 receptors arc expressed by Schivanii cells ill i,ivo. These results inclicate that ACh, by aiodulatiiig Schwaiin cell prolificratioii through M2 I-CCCPtOl- aclivatioa,)iiight cowributc to their progression to a niore dif rewiated phenotype.
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