Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 73, Issue 5, Pages 969-979Publisher
SPRINGER
DOI: 10.1007/s00253-006-0566-4
Keywords
polyhydroxyalkanoates; PHA synthase; genetic engineering; in vitro evolution; Escherichia coli; Ralstonia eutropha; Arabidopsis thaliana; PHA monomer-supplying enzymes
Categories
Ask authors/readers for more resources
Poly-3-hydroxyalkanoates [P(3HA)s] are biologically produced polyesters that have attracted much attention as biodegradable polymers that can be produced from biorenewable resources. These polymers have many attractive properties for use as bulk commodity plastics, fishing lines, and medical uses that are dependent on the repeating unit structures. Despite the readily apparent benefits of using P(3HA)s as replacements for petrochemical-derived plastics, the use and distribution of P(3HA)s have been limited by their cost of production. This problem is currently being addressed by the engineering of enzymes involved in the production of P(3HA)s. Polyhydroxyalkanoate (PHA) synthase (PhaC) enzymes, which catalyze the polymerization of 3-hydroxyacyl-CoA monomers to P(3HA)s, were subjected to various forms of protein engineering to improve the enzyme activity or substrate specificity. This review covers the recent history of PHA synthase engineering and also summarizes studies that have utilized engineered PHA synthases.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available