Journal
JOURNAL OF PROTEOME RESEARCH
Volume 6, Issue 7, Pages 2460-2471Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr060624p
Keywords
Rhodobacter; singlet oxygen; stress; regulation; protein synthesis; rpoE; sRNA
Categories
Ask authors/readers for more resources
Singlet oxygen (O-1(2)) is a stress factor and signal in the facultative phototrophic bacterium Rhodobacter sphaeroides. In vivo protein labeling with L-[S-35]-methionine and analysis by two-dimensional gel electrophoresis revealed that the synthesis of 61 proteins was changed in response to O-1(2). After O-1(2) treatment, protein synthesis patterns were distinct from those after H2O2 treatment but similar to those after high light exposure. This indicates regulatory mechanisms selective for different reactive oxygen species (ROS) and a response to light partly mediated by O-1(2). Analysis of mutant strains support that the response to O-1(2) is regulated mainly by rpoE (sigma(E)), but also a modulation of the sigma(E) dependent response by other factors and the existence of sigma(E) independent responses. The involvement of the RNA chaperon Hfq in the O-1(2) response implies a role of small regulatory RNAs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available