Journal
JOURNAL OF PROTEOME RESEARCH
Volume 6, Issue 5, Pages 2005-2010Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr060599n
Keywords
brain; proteomics; quantification; labeling; SILAM
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Funding
- NCRR NIH HHS [P41 RR11823-10, P41 RR011823-10, P41 RR011823] Funding Source: Medline
- NIMH NIH HHS [R01 MH067880, 5R01 MH067880-02, R01 MH067880-02] Funding Source: Medline
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR011823] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF MENTAL HEALTH [R01MH067880] Funding Source: NIH RePORTER
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We previously reported the metabolic N-15 labeling of a rat where enrichment ranged from 94% to 74%. We report here an improved labeling strategy which generates 94% N-15 enrichment throughout all tissues of the rat. A high N-15 enrichment of the internal standard is necessary for accurate quantitation, and thus, this approach will allow quantitative mass spectrometry analysis of animal models of disease targeting any tissue.
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