Journal
JOURNAL OF CELL BIOLOGY
Volume 176, Issue 1, Pages 35-42Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200605135
Keywords
-
Categories
Ask authors/readers for more resources
Rho GTPases participate in various cellular processes, including normal and tumor cell migration. It has been reported that RhoA is targeted for degradation at the leading edge of migrating cells by the E3 ubiquitin ligase Smurf1, and that this is required for the formation of protrusions. We report that Smurf1 dependent RhoA degradation in tumor cells results in the down-regulation of Rho kinase (ROCK) activity and myosin light chain 2 (MLC2) phosphorylation at the cell periphery. The localized inhibition of contractile forces is necessary for the formation of lamellipodia and for tumor cell motility in 2D tissue culture assays. In 3D invasion assays, and in in vivo tumor cell migration, the inhibition of Smurf1 induces a mesenchymal-amoebold-like transition that Is associated with a more Invasive phenotype. Our results suggest that Smurf1 is a pivotal regulator of tumor cell movement through Its regulation of RhoA signaling.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available