Journal
BIOLOGICAL & PHARMACEUTICAL BULLETIN
Volume 30, Issue 1, Pages 59-62Publisher
PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/bpb.30.59
Keywords
tetrandrine; cytokine; inducible nitric oxide synthase (iNOS); COX-2; THP-1 cell
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Tetrandrine (TET), a bis-benzylisoquinoline alkaloid isolated from the dried root of Hang-Fang-Chi (Stephania tetrandra S. MOORE), is traditionally used in China for treating inflammation, hypertension and silicosis. In this study, our aim was to examine the anti-inflammatory mechanism of TET through measuring the inducible nitric oxide synthase (iNOS), cyclooxygenase-1, and -2 (COX-1 and COX-2) expression, cytokines (TNF-alpha,, IL-4 and IL-8) formation, nitric oxide (NO) release and prostaglandin E-2 (PGE(2)) generation in lipopolysaccharide (LPS)-induced human monocytic (THP-1) cells. Results showed that TET remarkably suppressed the LPS (1 mu g/ml) induction of NO release and PGE(2) generation. It also significantly attenuated the LPS-induced transcription of proinflammatory cytokines (TNF-alpha, IL-4 and IL-8) in a dose-dependent manner. Furthermore, TET at 100 mu m significantly blocked the LPS induction of iNOS and COX-2 expression, but not the COX-1. Taken together, these results suggest that TET exerts anti-inflammatory effects probably through the suppression of COX-2 and iNOS expression.
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