Journal
ANALYTICAL BIOCHEMISTRY
Volume 360, Issue 1, Pages 63-74Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2006.10.013
Keywords
yeast two hybrid; gene expression; Saccharomyces cerevisiae; fiber-optic; imaging; spectroscopy; statistical analysis
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Yeast two-hybrid (Y2H) systems conventionally are used when screening for protein-protein interactions. A recurring problem with Y2H systems is the high prevalence of both false positives and false negatives. Distinguishing between real results and artifacts to characterize a protein-protein interaction properly can often be time-consuming and laborious. In this article, we report a new method for statistically evaluating single cell reporter gene transcriptional activation and expression in yeast two-hybrid systems. The system is based on statistical analysis of many yeast cells contained in a fiber-optic array, allowing for thorough characterization of reporter gene expression at different stringency levels. (c) 2006 Elsevier Inc. All rights reserved.
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