Journal
NATURE PROTOCOLS
Volume 2, Issue 1, Pages 38-41Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2007.15
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Here, we describe a Library screen transformation protocol using the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method is suitable for screening complex plasmid libraries such as those used for yeast two-hybrid analysis. This procedure takes up to 2.5 h to complete once the yeast culture has been grown.
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