Journal
NATURE PROTOCOLS
Volume 2, Issue 12, Pages 3166-3172Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2007.475
Keywords
-
Categories
Ask authors/readers for more resources
Circular dichroism (CD) is a useful technique for an assessment of DNA-binding mode, being a more accessible, low-resolution complement to NMR and X-ray diffraction methods. Ligand-DNA interactions can be studied by virtue of the interpretation of induced ligand CD signals resulting from the coupling of electric transition moments of the ligand and DNA bases within the asymmetric DNA environment. This protocol outlines methods to determine the binding mode and affinity of ligand-DNA interactions and takes approximately 7.5 h.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available