Journal
NATURE PROTOCOLS
Volume 2, Issue 2, Pages 287-295Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2006.478
Keywords
-
Categories
Funding
- Telethon [TCR07002] Funding Source: Medline
Ask authors/readers for more resources
Mitochondria participate in key metabolic reactions of the cell and regulate crucial signaling pathways including apoptosis. Although several approaches are available to study mitochondrial function in situ are available, investigating functional mitochondria that have been isolated from different tissues and from cultured cells offers still more unmatched advantages. This protocol illustrates a step-by-step procedure to obtain functional mitochondria with high yield from cells grown in culture, liver and muscle. The isolation procedures described here require 1 - 2 hours, depending on the source of the organelles. The polarographic analysis can be completed in 1 hour.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available