Journal
NATURE PROTOCOLS
Volume 2, Issue 11, Pages 3001-3008Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nprot.2007.448
Keywords
-
Categories
Ask authors/readers for more resources
Here, we describe a protocol for the selection of human antibody fragments using repertoires displayed on filamentous bacteriophage. Antigen-specific clones are enriched by binding to immobilized antigen, followed by elution and repropagation of phage. After multiple rounds of binding selection, specific clones are identified by ELISA. This article provides an overview of phage display and antibody technology, as well as detailed protocols for the immobilization of antigen, the selection of repertoires on purified or complex antigens and the identification of binders.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available