Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 68, Issue 3, Pages 639-642Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2006.11.008
Keywords
Nile Red (9-diethylamino-5H-benzo[alpha]phenoxazine-5-one); fluorescent probe; neutral lipids; microalgae; high-throughput screen
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Nile Red, a dye that fluoresces at defined wavelengths depending upon the polarity of the surrounding medium, has been proposed to determine the neutral lipid content of microalgal cells. Herein we communicate modifications to this technique that facilitate its use as a high-throughput screening technology, as well as improving its accuracy and versatility. (c) 2006 Elsevier B.V. All rights reserved.
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