4.3 Article

Fluorescent measurement of microalgal neutral lipids

Journal

JOURNAL OF MICROBIOLOGICAL METHODS
Volume 68, Issue 3, Pages 639-642

Publisher

ELSEVIER
DOI: 10.1016/j.mimet.2006.11.008

Keywords

Nile Red (9-diethylamino-5H-benzo[alpha]phenoxazine-5-one); fluorescent probe; neutral lipids; microalgae; high-throughput screen

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Nile Red, a dye that fluoresces at defined wavelengths depending upon the polarity of the surrounding medium, has been proposed to determine the neutral lipid content of microalgal cells. Herein we communicate modifications to this technique that facilitate its use as a high-throughput screening technology, as well as improving its accuracy and versatility. (c) 2006 Elsevier B.V. All rights reserved.

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