Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 12, Issue 2, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.2717531
Keywords
photoacoustic tomography; gene expression; molecular imaging; lacZ; reporter gene
Funding
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB000712] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS046214] Funding Source: NIH RePORTER
- NIBIB NIH HHS [R01 EB000712] Funding Source: Medline
- NINDS NIH HHS [R01 NS046214, R01 NS46214] Funding Source: Medline
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In the postgenomic era, imaging techniques are playing an important role in visualizing gene expression in vivo. This work represents the first demonstration of photoacoustic tomography (PAT) for reporter gene imaging. Rats inoculated with 9L/lacZ gliosarcoma tumor cells are imaged with PAT before and after injection of X-gal, a colorimetric assay for the lacZ-encoded enzyme beta-galactosidase. Using far-red optical illumination, the genetically tagged tumors in rats are clearly visualized by PAT following the assay. The spatial resolution is quantified to be less than 400 mu m, while 500-nM-level sensitivity is demonstrated. With the future development of new absorption-based reporter gene systems, it is anticipated that photoacoustic technology will provide a valuable tool for molecular imaging research. (C) 2007 Society of Photo-Optical Instrumentation Engineers.
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