Journal
GENOME BIOLOGY
Volume 8, Issue 9, Pages -Publisher
BMC
DOI: 10.1186/gb-2007-8-9-r203
Keywords
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Funding
- NIGMS NIH HHS [R01 GM067761] Funding Source: Medline
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM067761] Funding Source: NIH RePORTER
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Off-target effects have been demonstrated to be a major source of false-positives in RNA interference (RNAi) high-throughput screens. In this study, we re-assess the previously published transcriptional reporter-based whole-genome RNAi screens for the Wingless and Hedgehog signaling pathways using second generation double-stranded RNA libraries. Furthermore, we investigate other factors that may influence the outcome of such screens, including cell-type specificity, robustness of reporters, and assay normalization, which determine the efficacy of RNAi-knockdown of target genes.
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