Journal
LAB ON A CHIP
Volume 7, Issue 12, Pages 1783-1790Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b710524j
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Funding
- NIBIB NIH HHS [P41 EB002503, P41 EB002503-01] Funding Source: Medline
- NIGMS NIH HHS [R01 GM048027-10, R01 GM048027, GM48027] Funding Source: Medline
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [P41EB002503] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM048027] Funding Source: NIH RePORTER
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Asymmetrical delivery of stimuli to moving cells for perturbing spatially-heterogeneous intracellular signaling is an experimental challenge not adequately met by existing technologies. Here, we report a robust microfluidic platform allowing localized treatment of the front and/or back of moving cells which crawl through narrow channels that they completely occlude. The enabling technical element for this study is a novel design for precise, passive balancing of flow inside the microfluidic device by contacting two fluid streams before splitting them again. The microchannels constrain cell morphology and induce qualitative and quantitative changes in neutrophil chemotaxis that mimic cells crawling through tissues.
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