Journal
NATURE METHODS
Volume 4, Issue 1, Pages 35-37Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/NMETH977
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- Intramural NIH HHS Funding Source: Medline
- PHS HHS [N01-C0-12400] Funding Source: Medline
- NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH [ZIADE000714, ZIADE000380] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH [Z01DE000714, Z01DE000380] Funding Source: NIH RePORTER
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G protein-coupled receptors ( GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 mu g of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines. (c) 2007 Nature Publishing Group
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