Journal
CURRENT PHARMACEUTICAL DESIGN
Volume 13, Issue 2, Pages 173-183Publisher
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/138161207779313650
Keywords
cyanovirin; glycans; glycobiology; glycochip; high-mannose; HIV; microarray; scytovirin
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Funding
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [T32AI052070] Funding Source: NIH RePORTER
- NIAID NIH HHS [5T32 AI052070] Funding Source: Medline
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Progress in carbohydrate microarray technology has positioned the glycochip among the expanding set of biophysical tools available to researchers. Synthetically-derived glycochips unite established microarray techniques with the versatility and structural precision of synthetic carbohydrate chemistry. A comprehensive demonstration of carbohydrate microarrays is illustrated by the chip-based study of protein/carbohydrate and protein/glycoprotein interactions as they relate to HIV glycobiology. Composed of a series of high-mannose oligosaccharides, carbohydrate microarrays were prepared utilizing a covalent linking strategy to immobilize synthetically-defined glycans in a uniform orientation. In concert with a simple glycoprotein array, these microarrays were used to establish the individual and competitive binding profiles of five gp120 binding proteins-DC-SIGN, CD4, 2G12 cyanovirin-N, and scytovirin - and established the carbohydrate structural requirements for these interactions.
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