Journal
LAB ON A CHIP
Volume 7, Issue 5, Pages 588-595Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b703808a
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Funding
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R21HL084367] Funding Source: NIH RePORTER
- NHLBI NIH HHS [1R21HL084367-01A1] Funding Source: Medline
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A microfluidic device for continuous biosensing based on analyte binding with cytometric beads is introduced. The operating principle of the continuous biosensing is based on a novel concept named the particle cross over'' mechanism in microfluidic channels. By carefully designing the microfluidic network the beads are able to cross-over'' from a carrier fluid stream into a recipient fluid stream without mixing of the two streams and analyte dilution. After crossing over into the recipient stream, bead processing such as analyte-bead binding may occur. The microfluidic device is composed of a bead solution inlet, an analyte solution inlet, two washing solution inlets, and a fluorescence detection window. To achieve continuous particle cross over in microfluidic channels, each microfluidic channel is precisely designed to allow the particle cross over to occur by conducting a series of studies including an analogous electrical circuit study to find optimal fluidic resistances, an analytical determination of device dimensions, and a numerical simulation to verify microflow structures within the microfluidic channels. The functionality of the device was experimentally demonstrated using a commercially available fluorescent biotinylated fluorescein isothiocyanate (FITC) dye and streptavidin coated 8 mm-diameter beads. After, demonstrating particle cross over and biotin-streptavidin binding, the fluorescence intensity of the 8 mm-diameter beads was measured at the detection window and linearly depends on the concentration of the analyte (biotinylated FITC) at the inlet. The detection limit of the device was a concentration of 50 ng ml(-1) of biotinylated FITC.
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