Journal
JOURNAL OF NUTRIGENETICS AND NUTRIGENOMICS
Volume 1, Issue 1-2, Pages 30-48Publisher
KARGER
DOI: 10.1159/000109873
Keywords
Apoptotic gene expression; Calcitriol; Calcitriol/cortisone, microarray analysis; Cortisol; Gene expression, patterns; Gene expression, unique regulation; Human adipocyte proliferation; 11 beta-Hydroxysteroid dehydrogenase type 1; Oxidative and inflammatory stress; Real-time PCR
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Dietary calcium inhibits adiposity, and a key underlying mechanism is suppression of calcitriol, which modulates Ca2+ signaling and mitochondrial uncoupling in adipocytes. We demonstrated that calcitriol directly regulates adipocyte 11 beta-HSD-1 expression and cortisol production in human adipocytes in vitro and dietary calcium inhibits visceral adipose tissue 11 beta-HSD-1 expression in mice, indicating an interaction of calcitriol and cortisol in obesity. Consequently, we have evaluated the gene expression profile of human subcutaneous adipocytes treated with calcitriol and/or cortisone. Data analysis demonstrated significant calcitriol modulation of gene expression toward inhibition of the adipocyte apoptosis (e.g., VEGF and STC-2) and promotion of adipocyte proliferation (e.g., IGF-1 and IGF-1R). Calcitriol also up-regulated oxidative stress and inflammatory genes such as NOX-4 and TLR-3. The calcitriol/cortisone combination resulted in significant additional up-regulation of 11 beta-HSD-1 and down-regulation of adiponectin expression, while cortisone exerted little independent effect in the absence of calcitriol. Overall, calcitriol stimulated a pattern of adipocyte gene expression which favored adipocyte proliferation, oxidative and inflammatory stress and visceral adiposity, and these effects were amplified in the presence of cortisone; however, this conclusion must be tempered by the adipocyte source (subcutaneous) and requires confirmation in visceral adipocytes. Copyright (C) 2007 S. Karger AG, Basel
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