Journal
CANADIAN JOURNAL OF PLANT SCIENCE
Volume 88, Issue 2, Pages 313-322Publisher
CANADIAN SCIENCE PUBLISHING
DOI: 10.4141/CJPS07088
Keywords
Fragaria x ananassa; DNA fingerprinting; multivariate analysis; breeding; genetic similarity
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The goal of this study was to determine the level of genetic diversity and relatedness among 16 strawberry (Fragaria x ananassa Duch.) cultivars and 11 breeding lines developed in Canada, using Inter Simple Sequence Repeat (ISSR) markers. Seventeen primers generated 225 polymorphic ISSR-PCR bands. Cluster analysis by the unweighted pair-group method with arithmetic averages (UPGMA) revealed a substantial degree of genetic similarity among the genotypes ranging from 63 to 77% that were in agreement with the principal coordinate (PCO) analysis. Geographical distribution for the place of breeding program explained only 1.4%, of total variation as revealed by analysis of molecular variance (AMOVA). The ISSR markers detected a sufficient degree of polymorphism to differentiate among strawberry genotypes, making this technology valuable for cultivar identification and for the more efficient choice of parents in current strawberry breeding programs.
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