Journal
CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE
Volume 30, Issue 2, Pages 277-284Publisher
CANADIAN PHYTOPATHOLOGICAL SOC
DOI: 10.1080/07060661.2008.10540543
Keywords
Ustilago hordei; covered smut; barley; resistance gene; mapping
Categories
Funding
- Saskatchewan Agriculture Development Fund [19970258]
- Western Grains Research Foundation
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Covered smut of barley (Hordeum vulgare), caused by Ustilago hordei, is a seed-borne disease. To identify and map disease resistance genes, current Canadian barley cultivars, parents of six barley mapping populations, and four differentials namely 'Hannchen' (Rah1), 'Excelsior' (Ruh2). 'Plush' (Ruh6), and 'Odessa' (Universal susceptible), were evaluated. Six mated combinations of U. hordei sporidial mating harbouring knowing alleles of virulence (avr) and avirulence (Avr) genes were used in the cultivation. Most of the barley cultivars carried the Ruh1 resistance gene, but some also had Ruh2 or Ruh6. Many cultivars showed resistance to a U. hordei sporidial mating of genotype (avr1/avr1 avr2/avr-2 avr6/avr-6). Virulent on 'Hannchen'. 'Excelsior', and 'Plush'. The resistance indicated the presence of novel avirulence gene(s) corresponding to postulated resistance gene(s) in these cultivars. Fifty-six doubled-haploid lines from the mapping population 'Harrington'/TR306 were inoculated with a sporidial mating of genotype SM1 (Avr1/Avr1 avr2/avr2 avr6/avr6), avirulent on 'Hannchen' (Ruh1) but virulent on 'Excelsior' (Ruh2) and 'Plush' (Ruh6). 'Harrington' (ruh1) was susceptible to this fungal genotype possesing Avr1 and TR306 (Ruh1) was resistant. Based on field and greenhouse tests, barley lines seperated 27 resistant to 29 susceptible, which was not significantly different from a 1:1 ratio (chi(2) = 0.07, P = 0.79): this indicated the presence of the single major resistance gene Ruh1 in TR306. Ruh1 mapped to the short arm of chromosome 1 (7H) between markers iPgd1A and BCD129 on the 'Harrington'/TR306 map.
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