Journal
BIOPOLYMERS
Volume 90, Issue 3, Pages 450-458Publisher
WILEY
DOI: 10.1002/bip.20803
Keywords
protein splicing; protein alpha-thioester; expressed protein ligation; native chemical ligation; protein micro-arrays; chemoselective ligation; click chemistry; Staudinger ligation; site-specific protein derivatization
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Immobilization of proteins onto surfaces is of great importance in numerous applications, including protein analysis, drug screening, and medical diagnostics, among others. The success of all these technologies relies on the immobilization technique employed to attach a protein to the corresponding surface. Non-specific physical adsorption or chemical cross-linking with appropriate surfaces results in the immobilization of the protein in random orientations. Site-specific covalent attachment, on the other hand, leads to molecules being arranged in a definite, orderly fashion and allows the use of spacers and linkers to help minimize steric hindrances between the protein and the surface. The present work reviews the latest chemical and biochemical developments for the site-specific covalent attachment of proteins onto solid supports. (c) 2007 Wiley Periodicals, Inc.
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