Journal
ANALYTICAL METHODS
Volume 7, Issue 20, Pages 8942-8946Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ay02047f
Keywords
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Funding
- National Natural Science Foundation of China [61275085, 31100560]
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Due to the significant role of copper(II) ions in many biochemical and physiological processes, sensitive detection of copper(II) ions has attracted great attention. A novel quantitative copper(II) ion sensor is developed based on the conformational change of Cu2+ binding peptides using surface plasmon resonance (SPR) spectroscopy. The specific interaction between carboxyl groups of the peptide and Cu2+ induces a conformational change from alpha-helix to beta-sheet with decreasing hydrophilicity. The formation of Cu2+-peptide aggregates on the chip surface leads to a refractive index change, which results in a sensitive SPR signal change. Thus, Cu2+ can be measured selectively and sensitively due to the conformational change of Cu2+ specific binding peptides. With this well-designed sensing platform, the detection range of copper(II) ions is found to be 1 x 10(-12) M to 1 x 10(-6) M with a detection limit of 0.44 pM, which is 3 orders of magnitude lower than that in previous reports. The copper(II) ion sensor designed in this study is proposed for application in biological and environmental analysis.
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