Journal
AMERICAN JOURNAL OF ENOLOGY AND VITICULTURE
Volume 59, Issue 4, Pages 401-411Publisher
AMER SOC ENOLOGY VITICULTURE
Keywords
Saccharomyces; ethanol tolerance; ethanol utilization
Funding
- American Vineyard Foundation
- Maynard A. Amerine Endowment
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The yeast deletion set was screened to identify mutations impacting ethanol tolerance under limited aeration. Ethanol tolerance was correlated with growth and assessed as the ability to attain the final optical density reached by the parental strain. Of the 4,750 deletants evaluated, 175 strains showed a growth defect in the presence of 5% ethanol. Of these, 21 strains showed a severe growth defect. Growth of many of these strains improved with aeration, but five strains continued to show weak growth: CLC1 Delta, GSH1 Delta, UME6 Delta, TDP3 Delta, and VPS24 Delta. ADH1 Delta also showed higher levels of sensitivity to ethanol but did not grow as well as the other strains in the minimal medium used for the assay in the absence of ethanol and displayed a stronger effect of aeration. The deletion set was also screened to identify mutations affecting growth oil ethanol as sole substrate and energy source. Deletants were simultaneously evaluated oil lactate to define those mutations needed for growth oil respiratory substrates. A total of 176 deletants conferred a growth defect on both ethanol and lactate. Many of these genes are involved in respiration, in mitochondrial ATP synthesis, or define other mitochondrial functions required for organelle integrity. An additional 165 deletants resulted in an ethanol-specific growth defect. These deletants included other mitochondrial proteins as well as genes representing several different functional families. Only 14 genes conferred a specific growth defect oil lactate. No deletants were found to confer a growth defect on low sugar concentrations.
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