Journal
JOURNAL OF APPLIED PHYCOLOGY
Volume 20, Issue 3, Pages 315-321Publisher
SPRINGER
DOI: 10.1007/s10811-007-9255-7
Keywords
ethyl acetate extracts; growth inhibition; identification; Isochrysis galbana; isolation; microalgae
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A type of growth inhibitor was successfully isolated and purified from cell-free filtrates of cultural medium at the death phase of Isochrysis galbana, and its chemical structure was confirmed by the methods of FABMS, UV, H-1-NMR, C-13-NMR and 2D NMR, which was 1-[hydroxyl-diethyl malonate]-isopropyl dodecenoic acid, C22H38O7. The results showed that the growth-inhibitor strongly inhibited the growth of Isochrysis galbana, and the growth of the eight species of microalgae (Dunaliella salina, Platymonas elliptica, Chlorella vugralis, Nitzschia closterium, Chaetoceros muelleri, Chaetoceros gracilis, Nitzschia closterium minutissima, Phaeodactylum tricornutum) also could be regulated by the growth-inhibitor in a concentration-dependent manner. The further investigation found that the synthesis process of chlorophyll and protein in the cells of all test microalgae could be inhibited by the growth inhibitor, and the content of chlorophyll and protein significantly decreased.
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