Journal
CANADIAN JOURNAL OF MICROBIOLOGY
Volume 58, Issue 4, Pages 523-530Publisher
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/W2012-019
Keywords
dddP; mangrove soil; diversity; PCR; clone library
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Funding
- National Natural Science Foundation of China [30870448]
- Scientific Research Foundation for Returned Overseas Chinese Scholars, State Education Ministry
- BBSRC
- NERC of the United Kingdom
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The dddP gene encodes an enzyme that cleaves dimethylsulfoniopropionate (DMSP) into dimethyl sulfide (DMS) plus acrylate and has been identified in various marine bacteria and some fungi. The diversity of dddP genes was investigated by culture-independent PCR-based analysis of metagenomic DNA extracted from 4 mangrove soils in Southern China. A phylogenetic tree of 144 cloned dddP sequences comprised 7 groups, 3 of which also included dddP genes from previously identified Ddd+ (DMSP-dependent DMS production) bacteria. However, most (69%) of the DddP sequences from the mangroves were in 4 other subgroups that did not include sequences from known bacteria, demonstrating a high level of diversity of this gene in these environments. Each clade contained clones from all of the sample sites, suggesting that different dddP types are widespread in mangroves of different geographical locations. Furthermore, it was found the dddP genotype distribution was remarkably influenced by the soil properties pH, available sulfur, salt, and total nitrogen.
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