Journal
CANADIAN JOURNAL OF MICROBIOLOGY
Volume 58, Issue 5, Pages 678-681Publisher
CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS
DOI: 10.1139/W2012-030
Keywords
aspartic protease; Candida albicans; carboxypeptidase Y; nitrogen starvation; vacuole
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Funding
- Academy of Sciences of the Czech Republic [AV0Z40550506]
- Ministry of Education of the Czech Republic [LC 531]
- Czech Science Foundation [310/09/1945]
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Vacuoles play an important role in the physiology of pathogenic Candida spp. However, information on Candida albicans vacuolar enzymes, their properties, and regulation is scarce. Expression of the genes APR1 and CPY1 encoding vacuolar aspartic protease and serine carboxypeptidase, respectively, was analyzed using a clinical isolate of C. albicans. The transcription of both APR1 and CPY1 was upregulated in midexponential phase, together with increasing size of the vacuoles, when C. albicans was cultivated in yeast extract-peptone-dextrose agar at 30 degrees C. However, simultaneous upregulation of protein synthesis occurred only for Cpy1p. Analysis of APR1 and CPY1 expression under nitrogen-limited conditions revealed that the genes were regulated on both the transcriptional and translational levels and detectable amounts of Apr1p were synthesized only when C. albicans was grown in nitrogen-limited media.
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