Journal
CANADIAN JOURNAL OF MICROBIOLOGY
Volume 56, Issue 2, Pages 121-127Publisher
CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS
DOI: 10.1139/W09-110
Keywords
Trichoderma spp.; organophosphate pesticide stress; proteomics; 2-D SDS-PAGE
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Funding
- National High-Tech R&D Program (863 Program) of China [2006AA10Z409, 2006AA10A211]
- National Science and Technology Program of China [2006BAD08A06]
- Shanghai Rising Star Program [07QA14035]
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The proteomic approach is a powerful tool to study microbial response to environmental stress. To evaluate the responses of Trichoderma spp. to the organophosphate pesticide dichlorvos, mycelia of Trichoderma atroviride T23 were exposed to dichlorvos at concentrations of 0, 100, 300, 500, and 1000 mu g/mL, respectively. Changes in protein expression were investigated using two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. Sixteen protein spots were differentially expressed. They were identified by MALDI-TOF/TOF MS and were found to be linked to energy metabolism, transport, signal transduction, and stress tolerance. Among stress-related proteins, glutathione peroxidase-like protein (GPX), 1,4-benzoquinone reductase, and HEX1 were upregulated by and cyclophilin A induced by 1000 mu g/mL dichlorvos when compared with the control. These proteins were considered to be associated with fungal adaptation to adverse conditions. The results will help us to understand molecular mechanisms through which Trichoderma responds to organophosphate pesticides.
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