Journal
PLANT GROWTH REGULATION
Volume 54, Issue 1, Pages 71-79Publisher
SPRINGER
DOI: 10.1007/s10725-007-9226-7
Keywords
grape; sucrose transporter; LAHC; yeast; expression
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Large amounts of sugar are imported into grape berries from source leaves during ripening, and sucrose transporters play a key role during this process. In this study, a putative grape sucrose transporter gene VvSUC27, primarily expressed in sink tissue, was transformed into a yeast strain to characterize its function as a sucrose transporter. Sucrose was taken up by yeast transformed with VvSUC27 at an optimum pH of 4.0-5.0 and a K-m of 8.0-10.5 mM, indicating VvSUC27 is a LAHC (low-affinity/high-capacity) sucrose transporter. The ability of sucrose uptake in transformed yeast was activated by monosaccharides and inhibited by maltose and DEPC.
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