Journal
ANALYTICAL METHODS
Volume 7, Issue 8, Pages 3480-3485Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5ay00376h
Keywords
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Funding
- National Synergistic Innovation Center for Advanced Materials (SICAM)
- Postdoctoral Science Foundation of Jiangsu Province [1401032C]
- SRF for ROCS, SEM
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The present study aims to construct a biosensor to detect cholesterol by ChOx/HRP/Au/APTES/ITO. We tested the electrochemical characterization of this electrochemical sensor electrode using a cyclic voltammetry (CV) technique. Meanwhile, the electron transfer properties of the electrodes after different surface modifications were characterized by electrochemical impedance spectroscopy (EIS). This study demonstrated AuNPs as efficient electron transfer mediators for enzymes based on electrochemical cholesterol biosensors. The calibration linear relationship range of cholesterol was 0.5-450 mg dL(-1) with a sensitivity of 7.5 mu A mg(-1) dL(-1) cm(-2). The excellent performances of the developed biosensor indicated that it can act as a promising candidate for the electrochemical detection of cholesterol.
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