Journal
JOURNAL OF CYSTIC FIBROSIS
Volume 14, Issue 5, Pages 600-607Publisher
ELSEVIER
DOI: 10.1016/j.jcf.2015.05.005
Keywords
Adhesion molecules; Extracellular matrix; Porin D; Pseudomonas aeruginosa; Vitronectin
Categories
Funding
- Swedish Heart-Lung Foundation [20120622]
- Alfred Osterlund
- Anna and Edwin Berger
- Greta foundation
- Johan Kock foundation
- O. E. och Edla Johanssons
- Swedish Medical Research Council [521-2010-4221]
- Physiographical Society (Forssman's Foundation)
- Skane County Council's research and development foundation
- [NIH P30 DK089507]
- Novo Nordisk Fonden [NNF13OC0008399] Funding Source: researchfish
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Background: Pseudomonas aeruginosa is a pathogen that frequently colonizes patients with cystic fibrosis (CF) or chronic obstructive pulmonary disease (COPD). Several pathogens are known to bind vitronectin to increase their virulence. Vitronectin has been shown to enhance P. aeruginosa adhesion to host epithelial cells. Methods: We screened clinical isolates from the airways of CF patients and from the bloodstream of patients with bacteremia for binding of vitronectin. Two-dimensional SDS-PAGE and a proteomic approach were used to identify vitronectin-receptors in P. aeruginosa. Results: P. aeruginosa from the airways of CF patients (n = 27) bound more vitronectin than bacteremic isolates (n = 15, p = 0.025). Porin D (OprD) was identified as a vitronectin-binding protein. A P. aeruginosa oprD transposon insertion mutant had a decreased binding to soluble and immobilized vitronectin (p <= 0.001). Conclusions: P. aeruginosa isolates obtained from CF patients significantly bound vitronectin. Porin D was defined as a novel P. aeruginosa vitronectin-receptor, and we postulate that the Porin D-dependent interaction with vitronectin may be important for colonization. (C) 2015 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.
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