Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 74, Issue 5, Pages 1649-1652Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01864-07
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Funding
- NIAID NIH HHS [U19 AI056510, AI056510, AI057472, R01 AI057472] Funding Source: Medline
- NIGMS NIH HHS [GM 07739, T32 GM007739] Funding Source: Medline
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI057472, U19AI056510] Funding Source: NIH RePORTER
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A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample.
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