Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 46, Issue 1, Pages 286-289Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01172-07
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Funding
- FOGARTY INTERNATIONAL CENTER [D43TW006581] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [P01AI051976] Funding Source: NIH RePORTER
- National Institute on Minority Health and Health Disparities [T37MD001427] Funding Source: NIH RePORTER
- FIC NIH HHS [D43 TW006581] Funding Source: Medline
- NIAID NIH HHS [P01 AI51976, P01 AI051976] Funding Source: Medline
- NIMHD NIH HHS [T37 MD001427, 5 T37 MD001427] Funding Source: Medline
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Taeniasis due to Taenia solium is a disease with important public health consequences, since the larval stage is not exclusive to the animal intermediate, the pig, but also infects humans, causing neurocysticercosis. Early diagnosis and treatment of T. solium tapeworm carriers is important to prevent human cysticercosis. Current diagnosis based on microscopic observation of eggs lacks both sensitivity and specificity. In the present study, a nested-PCR assay targeting the Tso31 gene was developed for the specific diagnosis of taeniasis due to T. solium. Initial specificity and sensitivity testing was performed using stored known T. solium-positive and -negative samples. The assay was further analyzed under field conditions by conducting a case-control study of pretreatment stool samples collected from a population in an area (if endemicity. Using the archived samples, the assay showed 97% (31/32) sensitivity and 100% (123/123) specificity. Under field conditions, the assay had 100% sensitivity and specificity using microscopy/enzyme-linked immunosorbent assay coproantigen testing as the gold standards. The Tso31 nested PCR described here might be a useful tool for the early diagnosis and prevention of taeniasis/cysticercosis.
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