Journal
FEBS LETTERS
Volume 582, Issue 2, Pages 267-272Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2007.12.014
Keywords
melanoma; Docetaxel; class III beta-tubulin; apoptosis; taxanes
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We have previously shown that Docetaxel-induced variable degrees of apoptosis in melanoma. In this report, we studied the beta-tubulin repertoire of melanoma cell lines and show that class III beta-tubulin expression correlated with Docetaxel-resistance. Sensitive cells showed low levels of class III beta-tubulin with little microtubular incorporation, whereas class III beta-tubulin expression was higher in resistant cells and was incorporated into the cytoskeleton. As proof of concept, abrogation of class III by siRNA reverted Docetaxel-resistant cells to a sensitive phenotype, restoring the microtubular polymerisation response and promoting high levels of apoptosis through Bax activation. These results suggest that phenotypic expression of P-tubulin class III in melanoma may help identify patients with melanoma that can respond to taxanes. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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