Journal
BREAST CANCER RESEARCH AND TREATMENT
Volume 107, Issue 1, Pages 15-24Publisher
SPRINGER
DOI: 10.1007/s10549-007-9526-7
Keywords
biomarker; breast cancer; metabolism; tamoxifen; transforming growth factor beta
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Response to treatment with the antiestrogen tamoxifen is variable and at least partially due to its highly complex metabolism. Tamoxifen is transformed by polymorphic and inducible cytochrome P450 enzymes to a large number of metabolites with varying biological activities. The estrogen receptor dependent growth inhibitory effect of antiestrogens is mediated by activation of antiproliferative Transforming Growth Factor beta (TGF beta) signal transduction pathways. The aim of the present study was to establish if TGF beta 2 or TGF beta receptor II (T beta RII), could be used as markers to assess the pharmacological potency of tamoxifen and its metabolites. Consequently, we analyzed the growth inhibitory effect of tamoxifen and its major metabolites and explored whether it correlated with their capacity to induce TGF beta 2 and T beta RII expression. Human breast cancer cells (MCF-7 and T47D) were treated with tamoxifen and tamoxifen metabolites and mRNA expression of TGF beta 2 and T beta RII was analyzed by quantitative RT-PCR. Only two metabolites 4-hydroxytamoxifen and N-desmethyl-4-hydroxytamoxifen had significant antiproliferative activity and were able to induce TGF beta 2 and T beta RII. Plasma concentrations of these metabolites are usually very low in patients. However, even minor growth inhibitory effects at concentrations which are below the limit of quantification in plasma samples resulted in clearly discernible effects on expression of TGF beta 2 and T beta RII. Taken together, our data demonstrate that TGF beta 2 and T beta RII are very specific and sensitive biomarkers for the antiestrogenic activity of tamoxifen metabolites in breast cancer.
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