Journal
ONCOLOGY REPORTS
Volume 19, Issue 1, Pages 123-128Publisher
SPANDIDOS PUBL LTD
Keywords
glycosphingolipid; microdomain; cell-cell adhesion; E-cadherin; episialin; ether lipid 1-O-octadecyl-2-O-methyl-3-glycero-phosphocholine
Categories
Funding
- NATIONAL CENTER FOR RESEARCH RESOURCES [P20RR016480] Funding Source: NIH RePORTER
- NCRR NIH HHS [RR-16480] Funding Source: Medline
Ask authors/readers for more resources
The ether lipid 1-O-octadecyl-2-O-methyl-3glycero-phosphocholine (ET-18-OMe) inhibits cell-cell adhesion and induces invasiveness of breast cancer cells. Previously, we showed that a loss of cell-cell adhesion was due to sterical hindrance of E-cadherin by the anti-adhesive properties of the cell surface mucin episialin. Here, we demonstrated that the ether lipid ET-18-OMe induced the translocation of E-cadherin and episialin to membrane microdomains, enriched in glycosphingolipids, known to be involved in cell-cell adhesion and cell signaling. In addition, it was found that E-cadherin and clusters of episialin colocalized and associated with the glycosphingolipid, MSGb5, upon treatment with ET-18-OMe. Together, these results suggest that ET-18-OMe inhibits cell-cell adhesion by inducing the translocation of E-cadherin and episialin into MSGb5-enriched membrane microdomains, which leads to clustering and colocalization of the pro-adhesive E-cadherin and the anti-adhesive episialin thereby inhibiting cell-cell adhesion.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available