Journal
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
Volume 34, Issue 2, Pages 579-584Publisher
KOREAN CHEMICAL SOC
DOI: 10.5012/bkcs.2013.34.2.579
Keywords
PAMAM dendrimer; Gene delivery; L-Arginine; gamma-Aminobutyric acid (GABA)
Categories
Funding
- Basic Science Research Program through National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [20100021647]
- National Research Foundation of Korea
- Korean Government (MEST)
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In this study, we synthesized functional dendrimer derivatives as nonviral gene delivery vectors. Poly(amidoamine) dendrimer (PAMAM, generation 4) was modified to possess functional amino acids to enhance gene transfection efficiency. PAMAM G4 derivatives conjugated with L-arginine (Arg) and gamma-aminobutyric acid (GABA) showed higher transfection efficiency and lower cytotoxicity compared to the native PAMAM G4 dendrimer. The polyplex of the PAMAM 04 derivative/pDNA was evaluated using an agarose gel retardation assay and Picogreen reagent assay. Additionally, the MTT assay was performed to examine the cytotoxicity of synthesized polymers. All PAMAM 04 derivatives showed lower cytotoxicity than PEI25k.D. Particularly, PAMAM G4-GABA-Arg displayed enhanced transfection efficiency compared to the native PAMAM G4 dendrimer.
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