Journal
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
Volume 67, Issue 1, Pages 86-94Publisher
WILEY
DOI: 10.1111/j.1365-3083.2007.02042.x
Keywords
-
Categories
Ask authors/readers for more resources
With emergence of MHC class I tetramers loaded with CD8(+) T-cell viral epitopes, it is possible to study virus-specific CD8 cells in humans during infection and after vaccination. MHC class I tetramers was used to detect the frequency of haemagglutinin (HA)-specific T cells in 26 healthy influenza-vaccinated humans. Peripheral blood was collected before, and 7, 14 and 28 days after vaccination. Four-colour flow cytometry was used for monitoring of vaccine induced T-cell response. In 15 donors, two- to fivefold increase in frequency of HA-specific T cells was observed 7 days after vaccination. In addition, in 12 of these donors, this increase was accompanied with fourfold increase of H1N1 antibody titre. The increase in frequency of HA-specific CD8(+)/IFN-gamma(+) cells was low and peaked 28 days after vaccination in three of the six donors tested. Frequencies of HA-specific CD8(+) T cells and antibody titre returned to prevaccination values 1 year after vaccination. Subunit influenza vaccines have the ability to induce HA-specific CD8(+) cells. As the immune response to this vaccine decreased significantly after 1 year, our results confirm the importance of annual immunization for adequate protection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available