4.3 Article

Synthesis of Cysteine Capped Silver Nanoparticles by Electrochemically Active Biofilm and their Antibacterial Activities

Journal

BULLETIN OF THE KOREAN CHEMICAL SOCIETY
Volume 33, Issue 8, Pages 2592-2596

Publisher

KOREAN CHEMICAL SOC
DOI: 10.5012/bkcs.2012.33.8.2592

Keywords

Cys-AgNPs; Silver colloidal nanoparticles; (L)-Cysteine; Electrochemically active biofilm (EAB); Hydrogen bonding

Funding

  1. Brain Korea 21
  2. Korea Institute of Energy Technology Evaluation and Planning (KETEP) [20104010100580]
  3. Korean Ministry of Knowledge Economy
  4. Korea Evaluation Institute of Industrial Technology (KEIT) [20124030200100] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
  5. National Research Foundation of Korea [과C6B1910] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

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Cysteine capped silver nanoparticles (Cys-AgNPs) have been synthesized by employing electrochemically active biofilm (EAB), AgNO3 as precursor and sodium acetate as electron donor in aqueous solution at 30 C. Cys-AgNPs of 5-10 nm were synthesized and characterized by UV-Vis, FT-IR, XRD and TEM. Capping of the silver nanoparticles with cysteine provides stability to nanoparticles by a thiolate bond between the amino acid and the nanoparticle surface and hydrogen bonding among the Cys-AgNPs. In addition, the antibacterial effects of as-synthesized Cys-AgNPs have been tested against two pathogenic bacteria Escherichia coli (O157:H7) and Pseudomonas aeruginosa (PAO1). The results demonstrate that the as-synthesized Cys-AgNPs can proficiently inhibit the growth and multiplication of E. coli and P. aeruginosa.

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