Journal
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
Volume 32, Issue 6, Pages 2003-2007Publisher
KOREAN CHEMICAL SOC
DOI: 10.5012/bkcs.2011.32.6.2003
Keywords
Silver nanoparticle; Surface-enhanced raman scattering; Mercury ion; Aptamer sensor
Categories
Funding
- National Research Foundation of Korea [R11-2011-044-1002-0, K20904000004-09A050000410]
- KRIBB
- GRRC Program of Gyeonggi Province [GRRC-Hanyang-2010-CO3]
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We report a highly sensitive surface-enhanced Raman scattering (SERS) platform for the selective trace analysis of mercury (II) ions in drinkable water using aptamer-conjugated silver nanoparticles. Here, an aptamer designed to specifically bind to Hg(2+) ions in aqueous solution was labelled with a TAMRA moiety at the 5' end and used as a Raman reporter. Polyamine spermine tetrahydrochloride (spermine) was used to promote surface adsorption of the aptamer probes onto the silver nanoparticles. When Hg(2+) ions are added to the system, binding of Hg(2+) with T-T pairs results in a conformational rearrangement of the aptamer to form a hairpin structure. As a result of the reduced of electrostatic repulsion between silver nanoparticles, aggregation of silver nanoparticles occurs, and the SERS signal is significantly increased upon the addition of Hg(2+) ions. Under optimized assay conditions, the concentration limit of detection was estimated to be 5 nM, and this satisfies a limit of detection below the EPA defined limit of 10 nM in drinkable water
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